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Off-pathway product analysis of different metal ion-bound HsARDs. The enzymatic reaction mixture contained argon-saturated 50 mM HEPES pH 7.0, 1 mM MgCl2 buffer with 0.3 µM E1 enzyme, 250 µM E1 substrate in a total volume of 1 mL. About 100 µL of this reaction mixture was loaded onto the organic acid column. The quantitation is based on a 1 mL reaction mixture. The errors are represented as standard deviations of three replicates.
