Skip to main content
. Author manuscript; available in PMC: 2018 May 6.
Published in final edited form as: Biochem Biophys Res Commun. 2017 Mar 19;486(3):659–664. doi: 10.1016/j.bbrc.2017.03.086

Figure 2. Cyclic AMP increases hepatic expression of IP3R2.

Figure 2

Rat hepatocytes were isolated and then incubated with FSK (50 μM) plus IBMX (250 μM) or with 8-CPT-cAMP (0.2 mM) for 12 hrs. (a) Ip3r2 mRNA was measured by qPCR (*p<0.0001, n=8) and (b) IP3R2 was measured by densitometry of immunoblots (*p<0.001, n=5); Representative immunoblot shown in (c). (d) The increase in Ip3r2 message induced by cAMP was significantly lower in the presence of the mRNA synthesis inhibitor Actinomycin D (ACM; 1 μg/ml) for 5 hr (#p<0.05 by one-way ANOVA with Bonferroni’s post-tests, n=7).