Table 1.
Primer name | Sequence 5′--------------------------3′ |
---|---|
ITPR2 promoter cloning: | |
2 kb: | |
Forward | TCC CAG CTT CAA GCA ATT CTC |
Reverse | GGA CTA CAG CGG CCA AGA G |
1.5, 1.0 and 0.5 kb: | |
Forward -1.5 kb | CAA CAG AGA AGG CGT GGA AAA |
Forward -1.0 kb | GGA CGG GTC GAC TTT CAT T |
Forward – 0.5 kb | ACC TCT CCA GGG CTC CTC T |
Mutation of the CRE’s : | |
CRE1: | |
Forward | ACC CGC TGC GGC TTT CGC AGC CGC CCG GC |
Reverse | GCT CGC GTC CAC CTG GAA ATA AGC GGA CGG |
CRE3: | |
Forward | CCG TCC GCTTAT TTC CAG GTG GAC GCG AGC |
Reverse | CCG TCC GCT TAT TTC CAG GTG GAC GCG AGC |
CRE4: | |
Forward | CAA AAA CTG GCT TGG CCA AGT aAa aTT TAC CTC CTT GGA GGC TCG C |
Reverse | GCG AGC CTC CAA GGA GGT AAA ttT tAC TTG GCC AAG CCA GTT TTT G |
Primers for ChIP : | |
CRE1 ChIPF | CAC CTG GTC CAA AAA TTC CCC CTG A |
CRE1 ChIPR | CTC CAA CAC CTT TGC TCC TCC |
CRE2 ChIPF | CCA ACC GAT GTT TTT GAC CGA |
CRE2 ChIPR | CCG AAC TGG AGA TGA AAA G |
CRE3 ChIPF | GGC TGA AGG TTG GCT AAA AAC TG |
CRE3 ChIPR | GGC GTT AAT GAA ACT CGA CCC GT |
CRE2 ChIPF | CCC AGC TTT CAA GCA ATT CTG |
CRE2 ChIPR | GGC CAA CAT GGC GAA ACC CCA T |
CRE5 ChIPF | |
CRE5 ChIPR |
F – Forward; R- Reverse; ChIP: Chromatin Immunoprecipitation