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. Author manuscript; available in PMC: 2018 May 6.
Published in final edited form as: Biochem Biophys Res Commun. 2017 Mar 19;486(3):659–664. doi: 10.1016/j.bbrc.2017.03.086

Table 1.

Primer sequences used in this study

Primer name Sequence 5′--------------------------3′
ITPR2 promoter cloning:
2 kb:
Forward TCC CAG CTT CAA GCA ATT CTC
Reverse GGA CTA CAG CGG CCA AGA G
1.5, 1.0 and 0.5 kb:
Forward -1.5 kb CAA CAG AGA AGG CGT GGA AAA
Forward -1.0 kb GGA CGG GTC GAC TTT CAT T
Forward – 0.5 kb ACC TCT CCA GGG CTC CTC T
Mutation of the CRE’s :
CRE1:
Forward ACC CGC TGC GGC TTT CGC AGC CGC CCG GC
Reverse GCT CGC GTC CAC CTG GAA ATA AGC GGA CGG
CRE3:
Forward CCG TCC GCTTAT TTC CAG GTG GAC GCG AGC
Reverse CCG TCC GCT TAT TTC CAG GTG GAC GCG AGC
CRE4:
Forward CAA AAA CTG GCT TGG CCA AGT aAa aTT TAC CTC CTT GGA GGC TCG C
Reverse GCG AGC CTC CAA GGA GGT AAA ttT tAC TTG GCC AAG CCA GTT TTT G
Primers for ChIP :
CRE1 ChIPF CAC CTG GTC CAA AAA TTC CCC CTG A
CRE1 ChIPR CTC CAA CAC CTT TGC TCC TCC
CRE2 ChIPF CCA ACC GAT GTT TTT GAC CGA
CRE2 ChIPR CCG AAC TGG AGA TGA AAA G
CRE3 ChIPF GGC TGA AGG TTG GCT AAA AAC TG
CRE3 ChIPR GGC GTT AAT GAA ACT CGA CCC GT
CRE2 ChIPF CCC AGC TTT CAA GCA ATT CTG
CRE2 ChIPR GGC CAA CAT GGC GAA ACC CCA T
CRE5 ChIPF
CRE5 ChIPR

F – Forward; R- Reverse; ChIP: Chromatin Immunoprecipitation