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. Author manuscript; available in PMC: 2017 Sep 29.
Published in final edited form as: RSC Adv. 2016 Sep 29;6(98):95715–95721. doi: 10.1039/C6RA21226C

Figure 3.

Figure 3

A. Compounds 1 and 2 both bind 5FW Brd4 in the fast exchange regime. B. The binding isotherms showing the change in chemical shift of W81 upon titration of 1 and 2. Solid lines with filled symbols show the isotherms in the absence of Ni-DTPA; dashed lines with unfilled symbols indicate the presence of 20 mM Ni-DTPA. C,D. 1 and 2 were titrated into 50 µM 5FW Brd4, leading to a dose-dependent change in the chemical shift of the resonance corresponding to W81, located in the binding site.