Table 3.
Hematologic Parameters in Dimethyl Fumarate and Vehicle-Treated HbSS-Townes Mice
| Duration | ||||
|---|---|---|---|---|
| 1 week | 24 weeks | |||
| Treatment | ||||
| Vehicle | DMF | Vehicle | DMF | |
| Age (weeks) | 10 | 10 | 28 | 28 |
| F-Cells | n.d. | n.d. | 4.2 ± 1.3 | 8.8 ± 3.3a |
| Hematocrit (%) | 35.3 ± 3.8 | 34.8 ± 2.2 | 23.5 ± 2.6b | 22.0 ± 1.7b |
| Reticulocytes (%) | 37.2 ± 6.0 | 41.0 ± 4.0 | 57.6 ± 11.2b | 65.4 ± 11.5b |
| Irregular shaped RBC (%) | n.d. | n.d. | 25.3 ± 0.5 | 19.0 ± 1.4a |
| Total plasma heme (μM) | 46.6 ± 15.4 | 46.8 ± 13.2 | 39.0 ± 15.4 | 40.8 ± 12.0 |
| Plasma hemoglobin (g/L) | 0.14 ± .09 | 0.28 ± 0.17 | 0.24 ± 0.14 | 0.17 ± .06 |
| Plasma LDH (mU/ml) | 170 ± 7 | 170 ± 25 | 156 ± 21 | 135 ± 36 |
| Spleen (% body weight) | n.d. | n.d. | 6.8 ± 1.1 | 7.2 ± 1.0 |
| White counts (cells/μl ×10−3) | 32.7 ± 14.7 | 32.1 ± 13.3 | 24.9 ± 6.8 | 28.9 ± 10.4 |
HbSS-Townes mice were administered DMF (30 mg/kg/day; n = 4 mice) or vehicle (0.08% methyl cellulose; n = 4 mice) in drinking water for 24 weeks beginning at 4 weeks of age. Erythrocytes containing fetal hemoglobin (F-cells) were stained on blood smears by the Kleihauer–Betke method. Markers of hemolysis were measured, including packed RBC volume (hematocrit), reticulocytes, and spleen weight as a percentage of body weight. Values are mean ± SD.
a
p < 0.05, vehicle versus DMF
b
p < 0.05 10 weeks versus 28 weeks of age.
LDH, lactate dehydrogenase; n.d., not determined; RBC, red blood cells.