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HUVECs were pretreated with anti-RAGE (10 μg/mL) for 1 h or not, and then followed by LPS (1 μg/mL) stimulation for 24 h. (a) The TER was measured at 0, 12 and 24 h respectively. (b) The Pd value was measured at 12 and 24 h as described in Methods. The results are expressed as mean ± s.d. of three independent experiments. ** p < 0.01 vs. untreated control, & p < 0.01 vs. LPS.
