Fig. 1.

An increased genomic 5hmC level in IBDV infected bursa is associated with genomic fragmentation and increased expression of Tet1, Tet2 and immune responsive genes. Dot blot of bursal genomic DNA probed with a specific 5hmC antibody from either mock or IBDV UK661 infected RIR BF taken at various hours post infection (hpi) (A). Visualisation of genomic DNA integrity from mock or IBDV infected RIR BF by ethidium bromide. L = DNA size marker (B). Detection of IBDV VP2 capsid by western blot using protein lysates of RIR BF infected with IBDV UK661 at various time points (C). Fold changes of Tet1, Tet2, iNOS, CD40L and FasL gene expression within the RIR BF infected at various time points with IBDV as measured by real time RT-PCR (D). * = p < 0.05, ** = p < 0.01, *** = p < 0.001 as measured by Student unpaired t-test between mock and infected samples.