Figure 3.

Morphology changes of A549 cells induced by LY (acridine orange staining). A549 cells were inoculated into 6-well plates followed by addition of different concentrations of LY and cultured for 24 h. Acridine orange (final concentration of 1 μg/mL) was used to stain the cells for 15 min, and the cells were observed under a fluorescence microscope (×200). (A) 0 μM LY; (B) 1 μM LY; (C) 2 μM LY; (D) 4 μM LY; (E) 8 μM LY; (F) 16 μM LY; (G) 4 μM LY with 100 nM wortmannin; (H) 8 μM LY with 100 nM wortmannin; (I) 16 μM LY for 24 h, then added 100 nM wortmannin.