Table 5. The p values calculated using the Wilcoxon signed rank test for each gene in the three healthy donor cartilage discs in response to GDF5.
| A | Wildtype GDF5 | B | GDF5 variant | ||
|---|---|---|---|---|---|
| Gene | Time point | Gene | Time point | ||
| Day 3 | Day 7 | Day 3 | Day 7 | ||
| ACAN | 0.0078 (inc) | 0.50 | ACAN | 0.023 (dec) | 0.82 |
| COL2A1 | 0.50 | 0.25 | COL2A1 | 0.50 | 0.91 |
| SOX9 | 0.69 | 0.027 (inc) | SOX9 | 1.00 | 0.0039 (inc) |
| TIMP1 | 0.81 | 0.36 | TIMP1 | 0.94 | 0.16 |
| ADAMTS4 | 0.016 (inc) | 0.91 | ADAMTS4 | 0.13 | 0.019 (inc) |
| ADAMTS5 | 0.65 | 0.30 | ADAMTS5 | 0.22 | 0.039 (inc) |
| MMP1 | 0.22 | 0.43 | MMP1 | 0.94 | 0.74 |
| MMP13 | 0.31 | 0.016 (inc) | MMP13 | 0.0078 (dec) | 0.055 |
| BGLAP | 0.81 | 0.94 | BGLAP | 0.58 | 1.00 |
| COL1A1 | 0.012 (dec) | 0.38 | COL1A1 | 0.078 | 0.11 |
| COL10A1 | 0.13 | 1.00 | COL10A1 | 0.098 | 0.25 |
| IHH | 0.43 | 0.64 | IHH | 0.15 | 0.84 |
| RUNX2 | 0.58 | 1.00 | RUNX2 | 0.38 | 0.57 |
| RUNX2 bn | 0.69 | 1.00 | RUNX2 bn | 0.031 (inc) | 0.30 |
Data derives from Tables 3 and 4. Comparisons were between untreated (control) and treatment with either exogenous wildtype GDF5 (A) or with GDF5 variant (B). P values <0.05 and arising from fold changes in the same direction for all three donors are highlighted in bold. RUNX2 bn, bone specific RUNX2 transcript. (inc), increased expression; (dec), decreased expression.