Table 7. The fold change in expression of anabolic, catabolic and hypertrophic genes in the cartilage discs from each OA patient in response to variant A form of GDF5.
| Fold change in gene expression in response to variant A form of GDF5 in OA patients D-G | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Role | Gene | Day 3 | Day 7 | ||||||
| Patient D | Patient E | Patient F | Patient G | Patient D | Patient E | Patient F | Patient G | ||
| Anabolic | ACAN | 1.41 | 0.18 | 2.89 | 3.80 | 2.95 | 0.54 | 0.34 | 0.62 |
| COL2A1 | 2.53 | 0.26 | 7.31 | 8.14 | 2.13 | 0.37 | 0.32 | 0.50 | |
| SOX9 | 1.22 | 0.25 | 1.21 | 2.60 | 2.19 | 0.52 | 1.02 | 1.08 | |
| TIMP1 | 1.24 | 0.89 | 0.89 | 2.00 | 0.39 | 2.39 | 1.14 | 0.31 | |
| Catabolic | ADAMTS4 | 0.98 | 0.63 | 0.83 | 1.62 | 0.85 | 1.10 | 1.17 | 0.43 |
| ADAMTS5 | 0.51 | 3.23 | 0.29 | 2.25 | 0.25 | 2.67 | 5.26 | 0.64 | |
| MMP1 | 2.24 | 0.76 | 1.43 | 2.01 | 1.65 | 4.18 | 0.27 | 0.19 | |
| MMP13 | 3.81 | 1.76 | 1.45 | 3.38 | 0.57 | 1.87 | 5.23 | 0.35 | |
| Hypertrophic | BGLAP | 0.59 | 0.87 | 1.47 | 2.13 | 0.60 | 2.15 | 1.62 | 1.36 |
| COL1A1 | 0.75 | 0.54 | 0.54 | 1.55 | 1.84 | 0.84 | 0.87 | 0.94 | |
| COL10A1 | 0.69 | 1.69 | 1.20 | 4.77 | 1.03 | 1.46 | 0.61 | 0.62 | |
| IHH | 0.62 | 0.29 | 1.64 | 7.47 | 3.02 | 0.37 | 0.09 | 0.36 | |
| RUNX2 | 1.04 | 1.43 | 1.06 | 1.31 | 0.58 | 1.31 | 1.89 | 0.90 | |
| RUNX2 bn | 1.23 | 1.78 | 1.19 | 1.08 | 0.57 | 2.22 | 1.98 | 0.64 | |
Gene expression was measured at day 3 and day 7 of chondrogenesis without exogenous GDF5 (untreated) or with variant A form of GDF5. A fold change of 1 would represent no change in target gene expression relative to untreated. A value greater than 1 (highlighted in green) denotes increased gene expression relative to untreated and a value less than 1 (highlighted in red) denotes decreased gene expression relative to untreated. RUNX2 bn, the bone specific RUNX2 transcript.