Table 2. Oligonucleotides used in this study.

Name	Sequence (5′ → 3′)a	Purpose
OHS753	ATGTCTCCCAGACCACCAAGTATC	5′ vadA probe
OHS754	GGCTTGAGCTTGGATATGAACCG	3′ vadA probe
OHS859	GTGGTTGCTAGTCCGCAGAGAG	5′ flanking region of vadA
OHS860	GCTGGGTCAAACAAGCCAGTGC	3′ flanking region of vadA
OHS861	GGCTTTGGCCTGTATCATGACTTCA GGAGGCCGTAGTAGAGTGAAAGATG	5′ vadA with AfupyrG tail
OHS862	TTTGGTGACGACAATACCTCCCGAC CGACGAGCTGTATGCCTTCATG	3′ vadA with AfupyrG tail
OHS863	CTGGGTGGAGAGGCTAACTGC	5′ nested of vadA
OHS864	ACTCCAGCGCATTATCCAACTCAG	3′ nested of vadA
OHS887	ATATGAATTCATGTCTCCCAGACCACCAAGTATC	5′ vadA with EcoRI
OHS888	ATATGAATTCTGCTCCTGGTAAGAAATGCTCG	5′ vadA with pro and EcoRI
OHS889	ATATGCGGCCGCCAAGTTAGTGATGTACTCTTTCATATCC	3′ vadA with NotI
OJH84	GCTGAAGTCATGATACAGGCCAAA	5′ AfupyrG marker
OJH85	ATCGTCGGGAGGTATTGTCGTCAC	3′ AfupyrG marker
OJA142	CTGGCAGGTGAACAAGTC	5′ brlA probe
OJA143	AGAAGTTAACACCGTAGA	3′ brlA probe
OJA150	CAGTACGTCAATATGGAC	5′ wetA probe
OJA151	GTGAAGTTGACAAACGAC	3′ wetA probe
OJA156	ATGCTATATTCACCACCT	5′ rodA probe
OJA157	TGACCTACCAGAATATCG	3′ rodA probe
OMN66	TTTCCAGATCCTTCGCAG	5′ vosA probe
OMN63	ATAGAAACAGCCACCCAG	3′ vosA probe
OMN125	TATGCACTGGCACTCAAGCAACCG	5′ velB probe
OMN126	GTGCATGACGGTCGTATCTGGTCC	3′ velB probe
OMN143	ACTTATGCCAACGTTCTGCG	5′ fksA probe
OMN144	AAAGAGCGGGCAGCATAATG	3′ fksA probe
OMN176	CCATCACCATAAAGCGATCAG	5′ tpsA probe
OMN177	CAGTTTCGAGAAGTTAAGCGC	3′ tpsA probe
OMN182	CAGCCGCATCTCCAACTTAG	5′ orlA probe
OMN183	TGTTAGCAGCAATTCATGGCG	3′ orlA probe

^a Tail sequences are shown in italics. Restriction enzyme sites are in bold.