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. 2017 Feb 25;8(15):24046–24062. doi: 10.18632/oncotarget.15733

Figure 1. GFP-SCP binds and selectively internalizes into PSMA-overexpressing cells.

Figure 1

A. Schematic representation of GFP-SCP. B. LNCaP, PC3 and MCF7 cells were incubated with 25nM GFP-SCP for 5 h. The cells were fixed and stained with anti-GFP antibody (Cy3) and 4, 6-diamidino-2-phenylindole and viewed by laser scanning confocal microscopy. C. LNCaP and MCF7 cells were incubated with GFP-SCP as indicated, then subjected to flow cytometric analysis. D. LNCaP cells were monitored by laser confocal imaging, 0 to 72 min after the addition of 200nM GFP-SCP. Sulforhodamine-B was added to the medium immediately before adding the GFP-SCP, to mark the outside of the cells. The graph shows GFP fluorescence inside the cell, as measured using ImageJ.