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. 2017 Feb 25;8(15):24046–24062. doi: 10.18632/oncotarget.15733

Figure 2. Design, expression and purification of dsRB-SCP.

Figure 2

A. Schematic representation of dsRB-SCP. B. Expression and purification of dsRB-SCP: L: Cleared lysate, M: Molecular weight marker, E1: Eluate following IMAC (nickel sepharose column), E2: Purified dsRB-SCP eluted from IEX (ion exchange column). C. Binding of dsRB-SCP to dsRNA. dsRB-SCP (0.5-3μg) was preincubated with 500 bp long dsRNA in binding buffer at pH 8.3 or pH 5, and electrophoresed on a 2% agarose gel. M: 100 bp DNA molecular weight marker. Dashed lines indicate where the pictures of the gels were cut and reorganized.