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. 2017 Feb 25;8(15):24046–24062. doi: 10.18632/oncotarget.15733

Figure 5. dsRB-SCP/polyIC induces direct and PBMC-mediated bystander effects.

Figure 5

A. LNCaP-Luc/GFP cells were untreated or treated with dsRB-SCP/polyIC, polyIC alone or dsRB-SCP alone, as indicated. After 24 h, PBMCs were added to the test wells (black bars), and medium without PBMCs was added to the control wells (gray bars). The survival of LNCaP-Luc/GFP cells was measured using the Luciferase Assay System (Promega) (****P ≤ 0.0001, LNCaP-Luc/GFP+PBMCs vs LNCaP-Luc/GFP, ***P ≤ 0.001 dsRB-SCP/polyIC treatment vs polyIC alone). B. PC3-Luc/GFP cells co-cultured with PBMCs, in the presence or absence of LNCaP cells. PC3-Luc/GFP+LNCaP: LNCaP cells were untreated or treated with dsRB-SCP/polyIC, dsRB-SCP alone or polyIC alone, as indicated. After 24 h, PC3-Luc/GFP cells were added to the culture. 6 h later, PBMCs (black bars) or medium (hatched bars) was added to the culture. PC3-Luc/GFP: LNCaP growth medium, in the absence of cells, was untreated or treated with dsRB-SCP/polyIC, dsRB-SCP alone or polyIC alone, as indicated. After 24 h, PC3-Luc/GFP cells were added, and 6 h later either PBMCs (black bars) or medium (hatched bars) was added. The survival of PC3-Luc/GFP cells was measured using the Luciferase Assay System (Promega) (***P ≤ 0.001, PC3-Luc/GFP+LNCaP+PBMCs vs PC3-Luc/GFP+LNCaP; ****P ≤ 0.0001 PC3-Luc/GFP+LNCaP+PBMCs, dsRB-SCP/polyIC treatment vs polyIC alone; ***P ≤ 0.001, PC3-Luc/GFP+LNCaP+PBMCs vs PC3-Luc/GFP+PBMCs). Each experiment was conducted in triplicate. Representative data (mean and standard deviation) from three independent experiments are shown.