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. 2017 Mar 8;8(15):24063–24076. doi: 10.18632/oncotarget.16030

Figure 3. E2 induces FGF2 release in NSCLC cell lines and fulvestrant inhibits E2-induced FGF2 secretion.

Figure 3

In determining whether E2 treatment results in increased levels of FGF2 ligand release, cells were serum deprived for 48 hr prior to treatment with 10nM E2 or control for 1-4 hr. Media was collected and concentrated. Results were normalized to total protein content. A. 273T; B. 201T; C. A549. D. Serum-deprived A549 cells were pre-treated with 5μM fulvestrant for 24 hr followed by E2 treatment for 2 hr. Media and protein lysates were collected and analyzed for FGF2 secretion by ELISA. Results are expressed as the mean ± S.E. pg FGF2/mg protein released. *P < 0.05, **P < 0.01.