Figure 5. Phosphorylation of FRS2 in presence of fulvestrant and AZD4547.

201T and 273T cells were serum starved for 24 hr after plating, followed by addition of DMSO alone (control), AZD4547 (AZD; 2μM), fulvestrant (Fulv; 2μM), or the combination (A/F). Cells were harvested at 72 hr, whole cell lysates were immunoblotted for pFRS2. Expression of GAPDH was used as the loading control and densitometric measurements were corrected for amount of GAPDH.