Figure 3. CD8+CD122+PD-1+ Treg suppression of T cell proliferation.

in vitro. One-way MLR was set up using CD8+CD122+PD-1+ Tregs as suppressors, enriched T cells as responders or effectors (Teff), and irradiated Balb/C splenocytes as stimulators. The ratios of Treg to Teff were 1:4. In some groups, cell cultures were treated with anti-FasL or anti-IL-10 mAb, as described in the methods. T cell proliferation was analyzed using a thymidine-uptake method three (A.) and five (B.) days after the culture. Data are presented as mean ± SD. One representative of two separate experiments is shown.