Figure 4.

(A) Absorbance spectra of non-encapsulated PTX (20 μM, gray), BSA-HA conjugates at 1 mg protein/ml (solid black line), and of PTX in BSA-HA conjugates at 1 mg protein/ml (black, dashed line) compared to a mathematical summation (dotted line) of the absorbance spectra of non-encapsulated PTX and of the conjugate solution. DMSO concentration was 1.5 %. The molar ratio of PTX : BSA-HA conjugates was 2:1. (B) Particle size distribution determined using DLS, in solutions of BSA-HA conjugates (black, dashed line), non-encapsulated PTX (40 μM, gray) and of PTX-loaded BSA-HA NPs (solid black line) at the same concentrations of the respective components. The molar ratio of PTX:BSA-HA conjugates was 2:1.