Skip to main content
. 2017 Feb 21;8(15):24548–24563. doi: 10.18632/oncotarget.15595

Figure 2. ACE2 expression and activity in 5 groups of rats after gene transfer.

Figure 2

(A) ACE2 messenger ribonucleic acid (mRNA) expression was assayed by real time PCR in 5 groups of rats 2 weeks after gene transfer. (B) ACE2 activity. (C) Western blot analysis of the protein levels of ACE2 in homogenates of myocardium from five groups 2 weeks after gene transfer and (D) quantitative analysis of ACE2 protein expression in C. The blot is a representative of three blots from three independent experiments. (E) Immunohistochemistry (IHC) analysis of ACE2 of myocardium cross-section from each group rat 2 weeks after gene transfer and (F) quantitative analysis of ACE2 positive staining in E. Scale bar: 20 μm. *P < 0.05 vs. Control, Ad-ACE2 and Cilazapril. &P < 0.05 vs. Ad-ACE2. (G) Representative hematoxylin and eosin staining of myocardium cross-section in the five groups of rats 4 weeks after gene transfer. Scale bar: 20 μm. N is 8–15 in each group.