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. 2017 Feb 21;8(15):24548–24563. doi: 10.18632/oncotarget.15595

Figure 3. TUNEL staining and AMPK, caspase3, and Bcl-2 protein expression in five groups of rats 4 weeks after gene transfer.

Figure 3

(A) Apoptosis is detected by TUNEL staining in myocardial tissue sections from each group rat 4 weeks after gene transfer. Representative TUNEL staining images are shown. scale bar: 20 μm. (B) Quantitative analysis of TUNEL-positive staining cells. (C) The levels of pAMPK, AMPK, c-caspase3, caspase3 and Bcl-2 protein in myocardium were determined by Western blot and (D–F) quantitative analysis of pAMPK/AMPK, c-caspase3/caspase3 and Bcl-2 protein in C. The blot is a representative of three blots from three independent experiments. N is 8–15 in each group. *P < 0.05 vs. Control, Ad-ACE2 and Cilazapril. &P < 0.05 vs. Ad-ACE2.