Figure 6. Treatment of SJGBM2 cells with carmofur resulted in intracellular accumulation of ceramides.

Cell pellets were prepared and lipids were extracted for mass spectrometry as described in Materials and Methods. Intracellular ceramide and sphingolipid levels are from SJGBM2 cells (black bars) and SJGBM2 cells treated with 50 μM carmofur (gray bars). These levels were adjusted for equal loading based on the phosphate level. Results shown are mean ± SD of three replicates. *P < 0.05.