Skip to main content
. 2017 Feb 1;8(15):24762–24776. doi: 10.18632/oncotarget.14983

Figure 5. Loss of gankyrin in myeloid cells results in reduced expression of cytokines and stem cell markers.

Figure 5

A. RNA was extracted from non-tumor colon of tumor-harboring Mx1-Cre;Gankyrinf/f (Mx1-Cre;GKf/f) mice and Gankyrinf/f (GKf/f) mice. Relative amounts of mRNA were determined by real-time qPCR and normalized to the amount of actin mRNA. The amount of each mRNA in the untreated colon was given an arbitrary value of 1.0. Data are means ± SEM (n = 5). B. Effect of gankyrin on gene expression in colonic lamina propria (LP) cells. LP cells were isolated from DSS-treated Mx1-Cre;Gankyrinf/f (Mx1-Cre;GKf/f) mice and Gankyrinf/f (GKf/f) mice and cytokine mRNA expression was analyzed by real-time qPCR. The mRNA expression levels in LP cells from non-treated WT mice were set as 1. C. RNA was extracted from tumor tissues of Mx1-Cre;Gankyrinf/f (Mx1-Cre;GKf/f) mice and Gankyrinf/f (GKf/f) mice. Relative amounts of mRNA of ERK target genes cMyc, and stem cell markers Sox9, Bmi1, Lgr5 and Ascl2 were determined by real-time qPCR and normalized to the amount of actin mRNA. The amount of each mRNA in the untreated colon was given an arbitrary value of 1.0. Data are means ± SEM (n = 5). D. Relative amounts of mRNA of TNF-α and Pim1 from THP-1 cells transfected with gankyrin RNAi or control RNAi were determined by real-time qPCR and normalized to the amount of actin mRNA. The amount of each mRNA in the untreated colon was given an arbitrary value of 1.0. Data are means ± SEM (n = 3).