Figure 6. Suppression of the HCC growth after Plasmodium-based immunization.
(A) Measurement of tumor volumes at fourteen days after different immunization (RBCs, P.y-WT and P.y-GPC3 treated mice, n = 4/group). (B) Immunohistochemical staining (left) and assay quantification (right) for Ki-67 in different treated HCC tumors. Each symbol stands for the percentage of intended cells in an individual microscopic field in the scatter graph. Bar graphs show the mean percentage ± SD. Statistical differences between groups are indicated by the P values. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. Bars: 100 μm. Data are representative of four independent experiments.