Skip to main content
. 2017 Mar 1;8(15):24815–24827. doi: 10.18632/oncotarget.15820

Figure 5. Increased SRGN expression is accompanied by increased expression of a distinct set of mediators in both glioma cells and MCs.

Figure 5

(A) qPCR analysis for the expression of CXCL10, CXCL12 and TNF-α in the glioma cells after co-culture with LAD2 cells for 48 hours show a significant increase in expression in the co-cultured glioma cells as compared to not co-culturing with LAD2 cells. (B) qPCR analysis for the expression of CXCL1 and IL-6 in the LAD2 cells after co-culture with the glioma cells for 48 hours show a significant increase in expression in the co-cultured LAD2 cells as compared to not co-culturing with glioma cells. All the results are represented as increase in fold expression compared to the expression levels without co-culture. The experiments were performed 3 times, with triplicates in each case. Data is expressed as mean values + SEM and significance differences are indicated in the figure. GC = glioma cells, MC = LAD2 cells, GCMC = glioma cells co-cultured with LAD2 cells, MCGC = LAD2 cells co-cultured with glioma cells. ns = not significant, *p < 0.05, **p < 0.01 versus cells grown without co-culture.