Table 1. Human primers used for qPCR.
Target gene | Forward sequence | Reverse sequence |
---|---|---|
Beta actin | GGACTTCGAGCAAGAGATGG | AGCACTGTGTTGGCGTACAG |
ZEB1 | GGCATACACCTACTCAACTACGG | TGGGCGGTGTAGAATCAGAGTC |
SRGN | CGTCTGAGGACTGACCTTTTTCC | CGTTAGGAAGCCACTCCCAGAT |
Vimentin | GTTTCCCCTAAACCGCTAGG | GACACGGACCTGGTGGAC |
CD44 | CCAGAAGGAACAGTGGTTTGGC | ACTGTCCTCTGGGCTTGGTGTT |
MKI67 | GAAAGAGTGGCAACCTGCCTTC | GCACCAAGTTTTACTACATCTGCC |
CXCL10 | GGTGAGAAGAGATGTCTGAATCC | GTCCATCCTTGGAAGCACTGCA |
CXCL12 | CTCAACACTCCAAACTGTGCCC | CTCCAGGTACTCCTGAATCCAC |
TNF-α | CCCATGTTGTAGCAAACCCTC | TATCTCTCAGCTCCACGCCA |
IL-6 | GCCCTGAGAAAGGAGACAT | TTGTTTTCTGCCAGTGCCTC |
CXCL1 | AGCTTGCCTCAATCCTGCATCC | TCCTTCAGGAACAGCCACCAGT |