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. 2017 Feb 14;8(15):24915–24931. doi: 10.18632/oncotarget.15323

Figure 4. Transient knockdown of LEDGF/p75 sensitizes PC3-DR cells to clinically relevant taxanes DTX and CBZ.

Figure 4

A. LEDGF/p75 knockdown was confirmed by immunoblotting using a rabbit anti-LEDGF/p75 antibody in PC3-DR cells transfected with si-LEDGF/p75 oligos as compared to cells transfected with the siSD control oligos. B. Representative images of colony formation assay plates showing a decrease in clonogenicity in PC3-DR cells with LEDGF/p75 depletion compared to siSD control cells, in the presence and absence of drugs. Colonies were counted after 12 days of treatment. C. Bar graph showing quantification of PC3-DR colonies. Each bar represents the average of colonies counted in at least three independent experiments. SEM was calculated. D. Bar graph showing the percent clonogenicity in PC3-DR cells with LEDGF/p75 depletion, in the presence and absence of drugs, compared to untreated cells. Data was derived from the bar graph shown in panel C. SEM was calculated. Statistical significance was determined by comparing the values for cells transfected with siSD control oligos vs cells with LEDGF/p75 knockdown, in the presence or absence of drugs, using Student's t-test (*p<0.05).