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. 2017 Feb 15;8(15):24932–24948. doi: 10.18632/oncotarget.15326

Figure 4. 3-MA treatment restores the tumor growth rate in melanoma-bearing mice.

Figure 4

A. The tumor volumes in C57BL/6 mice (n=6/group) treated with saline (CON), 3-MA (3-MA), IR alone (IR) IR followed by 3-MA (IR+3-MA), IMQ combined with IR (IMQ+IR) or IMQ combined with IR and 3-MA (IMQ+IR+3-MA) were measured. Significant differences are indicated by **p < 0.01, and ***p < 0.001. B. Endogenous LC-3 expression in tumor tissue from six groups of tumors was determined via immunofluorescent staining. The tissues were stained with DAPI to visualize nuclei (blue) and were immunolabeled with an anti-LC3 antibody, which was detected via the addition of FITC-conjugated IgG (green). C. The number of cells containing LC3-positive puncta was counted under a microscope, and the percentage of cells containing LC3-positive puncta relative to the total cell number was calculated. The mean percentages ± SE from triplicate measurements are shown. Significant differences are indicated by **p < 0.01, and ***p < 0.001.