Figure 4. The p62^ΔUBA domain is responsible for the colocalization of LC3II and p62 in Tan I treated H28 cells.

A. Effect of Tan I on the colocalization of accumulated p62 with GFP-LC3II in H28 cells. B. The p62^ΔUBA domain is responsible for LC3II punctae formation by Tan I in H28 cells by immunofluorescence. H28 cells were transiently co-transfected with the Myc-p62^WT, Myc-p62^ΔUBA and Myc-p62^ΔLIR domain plasmids. The colocalization of LC3 and aggregates of full-length p62 or its deleted domain p62 plasmids was indicated by arrows. Bar scale =10 μm, GFP-LC3-Green, DAPI-blue, Myc-tagged p62-Red. C. Effect of Tan I on the expression of LC3II and p62 in H28 cells transfected with the Myc-p62^WT, Myc-p62^ΔUBA and Myc-p62^ΔLIR domain plasmids. Myc-tagged constructs of wild-type (WT) p62, p62^ΔUBA, and p62^ΔLIR were transfected into H28 cells in the presence or absence of Tan I, and Western blotting was performed with antibodies targeting p62, LC3II and β-Actin. An asterisk (*) indicates the p62 expression level of the Myc-tagged p62 constructs.