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. 2017 Feb 15;8(15):25032–25045. doi: 10.18632/oncotarget.15336

Figure 5. Tan I upregulates IRE1, CHOP and p-JNK in H28 cells.

Figure 5

A. Effect of Tan I on ER stress-related proteins such as GRP78, CHOP, IRE1 and PERK in two mesothelioma cell lines. Western blotting was performed to determine the effect of Tan I on the expression of GRP78, CHOP, PERK, ATF4, ATF6, IRE1 and β-Actin in H28 and H2452 cells. B. Time-dependent effect of Tan I on IRE1 and CHOP expression in H28 cells. C. Effect of Tan I on the phosphorylation of JNK in H28 and H2452 cells. Two mesothelioma cell lines were exposed to Tan I and Western blotting was performed to determine the effect of Tan I on JNK phosphorylation. D. Effect of the JNK inhibitor SP600125 on the expression of p62, LC3II and IRE1 in H28 cells. H28 cells were treated with Tan I (20 μM) in the presence or absence of SP600125 for 24 h. Western blotting was performed to determine the levels of LC3 conversion, and p62/SQSTM1, IRE1 and β-Actin expression.