Figure 3. Expression of angiogenesis markers in lungs of naïve and melanoma-carrying mice.

Total RNA was isolated from lungs of naïve mice (black bars) and lungs of mice taken day 14 post i.v. injection of B16F10 melanoma cells (gray bars). Total RNA was subjected to qPCR analysis of angiogenesis markers. Expression of genes was evaluated relative to GAPDH and normalized to naïve mice. Results are presented as mean ± SEM; n = 5 (black bars), n = 7-8 (gray bars). Note that mice with tumors do not show increased expression of angiogenesis markers compared to naïve mice and that the groups with tumors show similar expression of the markers. Statistical calculations were performed using the Mann Whitney test.