Table 2. Percentage (%) cells expressing β-tubulin III, SOX-2 and nestin in glioma primary cultures maintained in standard culture conditions (St-M) and in DMEM/F12 medium serum free with EGF, b-FGF and B27 supplement for neurosphere generation (GSC-M).
| β-tubulin III | SOX-2 | nestin | ||||
|---|---|---|---|---|---|---|
| St-M | GSC-M | St-M | GSC-M | St-M | GSC-M | |
| 1 | 92.35 ± 2.62 | 92.91 ±2.65 | 90.4 ± 4.52 | 97.84 ± 4.89 | 94.68 ± 3.73 | 95.92 ± 4.80 |
| 2 | 74.71 ± 3.74 | / | 14.56 ± 0.73 | / | 87.14 ± 2.36 | / |
| 3 | 80.1 ± 4.01 | / | 28.3 ± 1.42 | / | 79.98 ± 4.00 | / |
| 4 | 97.51 ± 4.88 | / | 8.01 ± 0.40 | / | 84.25 ± 4.21 | / |
| 5 | 38.77 ± 1.94 | / | 13.78 ± 0.69 | / | 43.25 ± 2.16 | / |
| 6 | 82.53 ± 2.13 | / | 35.99 ± 1.80 | / | 77.96 ± 3.90 | / |
| 7 | 90.77 ± 3.54 | 99.93 ±4.20 | 69.33 ± 3.47 | 84.01 ± 3.70 | 90.02 ± 4.50 | 99.99 ± 5.00 |
| 8 | 95.31 ± 4.77 | 98.86 ± 4.14 | 82.03 ± 4.10 | 84.71 ± 2.94 | 96.76 ± 4.84 | 99.35 ± 2.87 |
| 9 | 88.74 ± 1.44 | 94.78 ± 3.54 | 69.3 ± 3.47 | 80.30 ± 3.02 | 90.87 ± 3.54 | 99.63 ± 3.98 |
| 10 | 60.9 ± 3.05 | 81.30 ± 4.07 | 81.00 ± 3.05 | 65.73 ± 3.29 | 93.9 ± 2.70 | 95.40 ± 4.77 |
Data shown are presented as the mean values of one out of two independent experiments in duplicates ± SD.