Fig. 3.

Neuropeptide Y-based NPY (6–20) and NPY (21–36) egress HSPCs from the BM into the PB in ovariectomized mice. (A) Experimental design to determine the effects of NPY (6–20) and NPY (21–36) in ovariectomized mice. Twelve-week-old female WT mice were subjected to ovariectomy (OVX). One week after surgery, ovariectomized mice received an intraperitoneal injection with PBS, NPY (1–36), NPY (6–20), or NPY (21–36) for 21 days. (B) Body (left) and uterine (right) weights were measured to confirm the success of ovariectomy (n = 3–5 mice per group). (C and D) Expression levels of (C) HSPC maintenance factors in the BM and (D) the number of CFU cells in the PB of ovariectomized mice treated with PBS, NPY (1–36), NPY (6–20), or NPY (21–36) (n = 3 mice per group). (E) Flow cytometry analysis of LSK cells in the BM of each group (n = 5–7 mice per group). *P < 0.05. All error bars indicate S.E.M. All expression levels are relative to that of GAPDH mRNA.