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. 2017 Apr 7;17(4):794. doi: 10.3390/s17040794

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Sandwich immunodetection of PSA using Ab1-Au@MNPs, magnetic collection and enzymatic transduction; (B) Calibration plots of reduction current versus PSA concentration using planar Ab₁-modified gold electrode (black dots) and Ab₁-Au@MNPs (black dots), anti-biotin immobilised on thioctic acid modified Au@MNPs (red squares) and thioctic acid modified Au@MNPs exposed to Ab₁ without EDC/NHS activation (blue triangles). Reproduced from [146] with permission from The Royal Society of Chemistry. (C) Steps required for (a) the magnetic capture of PSA using Ab₁-Au@MNPs; (b) construction of the GO-based label; and (c) sandwich immunodetection of CEA; (D) Calibration plots of the magnetic immunoassay toward CEA standards in pH 5.3 acetic acid salt-buffered saline buffer + 3.0 mM H₂O₂. Inset: corresponding linear and DPV curves. Reprinted from [147], Copyright 2012, with permission from Elsevier.

(A) Sandwich immunodetection of PSA using Ab1-Au@MNPs, magnetic collection and enzymatic transduction; (B) Calibration plots of reduction current versus PSA concentration using planar Ab₁-modified gold electrode (black dots) and Ab₁-Au@MNPs (black dots), anti-biotin immobilised on thioctic acid modified Au@MNPs (red squares) and thioctic acid modified Au@MNPs exposed to Ab₁ without EDC/NHS activation (blue triangles). Reproduced from [146] with permission from The Royal Society of Chemistry. (C) Steps required for (a) the magnetic capture of PSA using Ab₁-Au@MNPs; (b) construction of the GO-based label; and (c) sandwich immunodetection of CEA; (D) Calibration plots of the magnetic immunoassay toward CEA standards in pH 5.3 acetic acid salt-buffered saline buffer + 3.0 mM H₂O₂. Inset: corresponding linear and DPV curves. Reprinted from [147], Copyright 2012, with permission from Elsevier.