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. 1976 Aug;58(2):237–239. doi: 10.1104/pp.58.2.237

Metabolism As a Function of Water Potential in Air-Dry Seeds of Charlock (Sinapis arvensis L.) 1

Miriam Edwards a
PMCID: PMC542219  PMID: 16659654

Abstract

A new method is described for studying the metabolism of air-dry seeds. An initial pulse of 14CO2 was supplied to seeds maintained in air at controlled low water potentials for 6 months. Seeds were also infiltrated with 2-14C-acetate and with 14C-l-leucine at 0 C, redried rapidly at 0 C, and maintained at controlled low water potentials for 4 to 6 weeks. The metabolism of the air-dry seeds was a function of the water content of the tissues, which was in equilibrium with the water potential at the seed surface. The fixation of 14CO2 and the utilization of 2-14C-acetate increased exponentially with water content. The incorporation of 14C-l-leucine into protein increased linearly with water content. Metabolism was not reduced to a low rate except in air-dry seeds at the lowest water potentials (−1716 to −762 bars) with 4 to 6% water.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abdul-Baki A., Baker J. E. Changes in Respiration and Cyanide Sensitivity of the Barley Floret during Development and Maturation. Plant Physiol. 1970 Jun;45(6):698–702. doi: 10.1104/pp.45.6.698. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CANVIN D. T., BEEVERS H. Sucrose synthesis from acetate in the germinating castor bean: kinetics and pathway. J Biol Chem. 1961 Apr;236:988–995. [PubMed] [Google Scholar]
  3. Ihle J. N., Dure L. S., 3rd The developmental biochemistry of cottonseed embryogenesis and germination. 3. Regulation of the biosynthesis of enzymes utilized in germination. J Biol Chem. 1972 Aug 25;247(16):5048–5055. [PubMed] [Google Scholar]
  4. Marcus A. Seed germination and the capacity for protein synthesis. Symp Soc Exp Biol. 1969;23:143–160. [PubMed] [Google Scholar]
  5. Ragai H., Loomis W. E. Respiration of Maize Grain. Plant Physiol. 1954 Jan;29(1):49–55. doi: 10.1104/pp.29.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Roberts B. E., Paterson B. M. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330–2334. doi: 10.1073/pnas.70.8.2330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Roberts B. E., Payne P. I., Osborne D. J. Protein synthesis and the viability of rye grains. Loss of activity of protein-synthesizing systems in vitro associated with a loss of viability. Biochem J. 1973 Feb;131(2):275–286. doi: 10.1042/bj1310275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Yoo B. Y. Ultrastructural changes in cells of pea embryo radicles during germination. J Cell Biol. 1970 Apr;45(1):158–171. doi: 10.1083/jcb.45.1.158. [DOI] [PMC free article] [PubMed] [Google Scholar]

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