Table-1.
Names and sequences of the PCR primer pairs used.
| PCR reaction | Primer name and sequence | Annealing temperature (°C) | Product length (bp) | Restriction enzyme |
|---|---|---|---|---|
| 5’SAG2 primary PCR | SAG2.F4: (5’-GACCTCGAACAGGAACAC-3’) SAG2.R4 (5’-GCATCAACAGTCTTCGTTGC-3’) |
61 | 300 | Sau3AI |
| 5’SAG2 secondary PCR | SAG2.F: (5’-GAAATGTTTCAGGTTGCTGC-3’) SAG2R2: (5’-GCAAGAGCGAACTTGAACAC-3’) |
61 | 241 | |
| 3’SAG2 primary PCR | SAG2.F3: (5’-TCTGTTCTCCGAAGTGACTCC-3’) SAG2.R3: (5’-TCAAAGCGTGCATTATCGC-3’) |
63 | 350 | HhaI |
| 3’SAG2 secondary PCR | SAG2.F2: (5’-ATTCTCATGCCTCCGCTTC-3’) SAG2.R: (5’-AACGTTTCACGAAGGCACAC-3’) |
63 | 221 | |
| GRA6 primary PCR | GRA6.FO: (5’-GGCAAACAAAACGAAGTG-3’) GRA6.RO: (5’-CGACTACAAGACATAGAGTG-3’) |
55 | 950 | MseI |
| GRA6 secondary PCR | GRA6.F: (5’-GTAGCGTGCTTGTTGGCGA-3’) GRA6.R: (5-TACAAGACATAGAGTGCCCC-3) |
58 | 750 |
PCR=Polymerase chain reaction