Supplementary Table-S3.
Standard curve data from real-time PCRs of bovine neutrophils.
| Gene | Log dilutions | Ct | Correlation coefficient (R2) | Equation of standard curve |
|---|---|---|---|---|
| MIRLET7E | 10−1-10−6 | 10-23 | 0.9996 | Y=−2.538*X+25.67 |
| MIR17 | 10−1-10−5 | 16-32 | 0.9929 | Y=−4.045*X+37.10 |
| MIR24-2 | 10−1-10−6 | 7-23 | 0.9944 | Y=−3.093*X+26.89 |
| MIR146A | 10−1-10−5 | 9-33 | 0.9972 | Y=−5.985*X+39.72 |
| MIR181C | 10−1-10−6 | 8-23 | 0.9934 | Y=−3.013*X+26.65 |
| MIR16A | 10−1-10−6 | 7-24 | 0.9986 | Y=−3.409*X+27.52 |
| IL1B | 10−1-10−5 | 8-20 | 0.9956 | Y=−3.147*X+24.33 |
| IL6 | 10−1-10−5 | 10-25 | 0.9964 | Y=−3.759*X+29.47 |
| CXCL8 | 10−1-10−5 | 9-21 | 0.9775 | Y=−2.945*X+25.38 |
| TLR4 | 10−1-10−5 | 8-24 | 0.9860 | Y=−3.979*X+29.05 |
| TNF | 10−1-10−5 | 10-26 | 0.9991 | Y=−4.100*X+30.73 |
| ACTB | 10−1-10−5 | 11-26 | 0.9953 | Y=−3.611*X+29.76 |
IL1B=Interleukin 1β, TNF=Tumor necrosis factor, TLR4=Toll-like receptor 4, PCR=Polymerase chain reaction, CXCL8=Chemokine (C-X-C motif) ligand 8, IL6=Interleukin 6, ACTB=Beta-actin