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. 1976 Sep;58(3):315–319. doi: 10.1104/pp.58.3.315

Cytochemical Localization of Glycolate Dehydrogenase in Mitochondria of Chlamydomonas1

Belinda B Beezley a, Peter J Gruber a, Sue Ellen Frederick a
PMCID: PMC542238  PMID: 16659670

Abstract

Mildly disrupted cells of Chlamydomonas reinhardi Dangeard were incubated in a reaction medium containing glycolate, ferricyanide, and cupric ions, and then processed for electron microscopy. As a result of the cytochemical treatment, an electron opaque product was deposited specifically in the outer compartment of mitochondria; other cellular components, including microbodies, did not accumulate stain. Incubation with d-lactate yielded similar results, while treatment with l-lactate produced only a weak reaction. Oxamate, which inhibits glycolate dehydrogenase activity in cell-free extracts, also inhibited the cytochemical reaction. These findings demonstrate in situ that glycolate dehydrogenase is localized in mitochondria, and thus corroborate similar conclusions reached on the basis of enzymic studies of isolated algal organelles.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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