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. 2017 May 9;11:133. doi: 10.3389/fncel.2017.00133

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Copyright © 2017 Hatsukano, Kurisu, Fukumitsu, Fujishima and Kengaku.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Molecular perturbation of PGC-1α inhibits dendritic outgrowth and mitochondrial activity in Purkinje cells. (A) Representative images of Purkinje cells overexpressing EGFP (control), EGFP-NRF1DN or FLAG-RIP140. Cells were stained for Calbindin at 10 DIV. Scale bar, 20 μm. (B–D) Quantitative analyses of the total dendritic length (B), number of dendritic branches (C) and PDH signal (D). N = 40 cells for control, 30 cells for NRF1DN and 30 cells for RIP140. Data represent mean ± SEM, **p < 0.01, one-way ANOVA followed by Tukey Kramer HSD tests.