Figure 8.

PGC-1α overexpression enhances dendritic outgrowth of Purkinje cells in the absence of T3. (A,B) The morphology of Purkinje cells transfected with tdTomato (control) or PGC-1α-mCherry (+PGC-1α). Cells were cultured with (B) or without (A) T3 and stained for Calbindin at 10 DIV. Scale bars, 20 μm. PGC-1α overexpression induced dendritic outgrowth in the absence of T3 (A), but not in the presence of T3 (B). (C–E) Quantitative analyses of total dendritic length (C) branch numbers (D) and PDH signal (E). Purkinje cells expressing tdTomato (control) or PGC-1α were cultured with or without T3 treatment. N = 30 for all data points. Data represent mean ± SEM, ***p < 0.001 and *p < 0.05, Student’s t test.