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. 2017 May 9;8:794. doi: 10.3389/fmicb.2017.00794

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Copyright © 2017 Bedrunka and Graumann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dynamics and simultaneous localization of YdaK and DgcK in B. subtilis NCIB3610. (A) Representative time-lapse kymographs of YdaK-mV-YFP (left panel, strain NCIB3610-PB57; amyE::P_xyl-ydaK-mV-yfp) and DgcK-mV-YFP (right panel, strain NCIB3610-PB90; amyE::P_xyl-dgcK-mV-yfp) 45 min after induction with 0.1% xylose (v/v). BF: bright field (first row); snapshots (second row) and maximum intensity projection (MIP, third row) from time-lapse microscopy; fourth row: kymographs of fluorescence intensities along the rectangular selection depicted in the third row. Images were taken every 0.1 s upon continous illumination with 515 nm. (B) Co-localization of DgcK-CFP (445 nm, false colored red) originated from the ectopic amyE locus and YdaK-mV-YFP (515 nm, false-colored green) produced from the original locus, triangles indicate co-localization events (strain NCIB3610-PB37-PB10; amyE::P_xyl-dgcK-cfp, P_ydaK-ydaK-mV-yfp). Scale bars: 2 μm.