Figure 2.

ADE of DENV replication in K562 cells mediated by prM mAb. prM mAb was diluted to a series of different concentrations, then they were incubated with DENV-1 for 1 h at 25°C and transferred to K562 cells at MOI of 1. Infected cells were incubated for 72 h at 37°C. (A) Viral RNA copies in infected supernatant were quantified by qRT-PCR. (B) Infected cells were determined by flow cytometry. Data are expressed as means of three independent experiments. Error bars show the means ± SEM.