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. 2017 May 9;7:157. doi: 10.3389/fcimb.2017.00157

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Copyright © 2017 Wang, Yang, Huang, Huang, Zhang, Wang, Zhang and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of prM-AIDs. (A) The titer of prM-AIDs in immunizzed sera were determined by ELISA every 2 weeks (n = 6). Dilution of immunized or naive mouse sera were coated in 96-well plates. Biotinylated prM mAb were added. Bound prM mAb was detected by addition of streptavidin-HRP. (B) Purified prM-AIDs was identified by SDS-PAGE. (C) The specificity of prM-AIDs was analyzed by ELISA. Diluted naive mouse antibodies (NM-Abs), or immunized mouse antibodies (IM-Abs) were coated in 96-well plates. Biotinylated prM mAb were added. Bound prM mAb was detected by addition of streptavidin-HRP. Dotted line shows samples were considered positive if Positive/Native ration>2.1.