Figure 3.

Type of cell death induced by viral oncolysis of JX-GFP and TG6002 in SK29-MEL-1.

Notes: Cell death was analyzed by flow cytometry. Cells were infected with JX-GFP or TG6002 using an MOI of 0.0001 and combined with 5 d of treatment with 100 µg/mL 5-FC or 50 µg/mL 5-FU. Cells were harvested and stained with Annexin V and propidium iodide and measured via flow cytometry. Data are shown for at least two independent experiments. (A) Overview of all cells. (B) Annexin V positive representing cells in early apoptosis. (C) Propidium iodide-positive cells representing cells undergoing necrosis. (D) Annexin V and propidium iodide-positive cells representing cells in late apoptosis. (E) Representative dot plots of untreated SK29-MEL-1 and TG6002 or JX-GFP-treated cells using quadrant gating. X-axis = propidium iodide (PI) and Y-axis = Annexin APC labeled.

Abbreviations: MOI, multiplicity of infection; d, day; 5-FC, 5-fluorcytosin; 5-FU, 5-fluoruoracil.