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. 2017 May 4;10:2413–2424. doi: 10.2147/OTT.S130087

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© 2017 Sun et al. This work is published and licensed by Dove Medical Press Limited

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(A) ECTCs possessing only epithelial molecular markers (EpCAM and cytokeratin 8/18/19) were stained with red immunofluorescence. (B) EMCTCs having both epithelial and mesenchymal molecular markers were stained with both green and red immunofluorescent dyes. (C) MCTCs occupying only mesenchymal molecular markers (vimentin and Twist) were stained with green immunofluorescence. (Bars =2.5 µm).

Abbreviations: ECTCs, epithelial circulating tumor cells; EMCTCs, epithelial–mesenchymal circulating tumor cells; EpCAM, epithelial cell adhesion molecule; MCTCs, mesenchymal circulating tumor cells.