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Time-lapse confocal imaging of the equatorial plane of (A) phase separated GUVs (DOPC:SSM:Chol:GM1 = 40:40:20:0.1) and (B) phase separated GPMVs prepared from RBL cells (labeled with the Ld marker Fast-DiI, red), following the addition (at time 0) and rapid binding of Alexa 488-labeled CTxB (green) to GM1. Binding occurs right after CTxB addition in the Lo phase. Scale bar 10 μm. Experiments were done at 10°C. Miscibility temperature for GPMVs is around 15°C.
