FIGURE 4.

5-HETE promotes SS and germling formation. Quantification of SS and germlings (GL) for loxB strains grown in GMM +0.5% tergitol (mock) (A), 0.5 μg/μL AA (B) or 5-HETE (C) or both (D). While exposure to AA reduces the number of SS and GLs compared to wild type in the ΔloxB strain, exposure to 0.5 μg/μL 5-HETE, or AA and 5-HETE maintains or increases the proportion of SS and GLs compared to the mock treatment, respectively. (E) SS are significantly increased compared to the mock treatment in the presence of AA and 5-HETE. Few SS were observed in the OE::loxB strain since all DSs had already progressed to the GL stage (F). Values represent average of n = 3 ± SEM and Student’s t-test was used to identify statistical differences, ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.