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. 2017 May 9;10:139. doi: 10.3389/fnmol.2017.00139

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Copyright © 2017 Wang, Zhang, Chen, Liu, Teng, Zhao and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Asct2 mRNA expression was regulated by histone acetylation but not DNA methylation in the promoter. (A–C) Acetylation of histones H3K4 (A), H3K9 (B), and H3K27 (C) in three different regions of the Asct2 promoter (triplicate in RT-qPCR repeat, ^*p < 0.05, CSDS vs. control mice; unpaired t-test). (D) Percentage of methylated clones at different CpG sites in the Asct2 promoter (black in every circle represents the percentage of methylation in each CpG; TSS, transcription start site). (E) Relative Asct2 mRNA expression in HT22 hippocampal cells treated with 5 or 10 mM suberoylanilide hydroxamic acid (SAHA) (triplicate in RT-qPCR repeat, ^*p < 0.05, one-way ANOVA). (F) H3K9 acetylation at the P2 Asct2 promoter region in SAHA-treated HT22 cells (^*p < 0.05 and ^***p < 0.001, 10 mM SAHA vs. vehicle; unpaired t-test). (G) A diagram shows the locations of the primers used in CHIP assay and sequenced part in bisulfite sequencing.