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. 2017 May 9;10:139. doi: 10.3389/fnmol.2017.00139

Figure 5.

Figure 5

D-serine level in the hippocampus was regulated by ASCT2. (A–C) Co-localization of D-serine and ASCT2 in the hippocampus: D-serine (A); ASCT2 (B); Merge (C). (D) Relative levels of L-serine, D-serine, and L-glutamine in the hippocampus of control (Con) and CSDS mice infected by lentiviruses carrying Asct2-specific shRNA (shASCT2) or scrambled shRNA (shSRC) (n = 4, *p < 0.05, two-way ANOVA). (E) mRNA expression of Asct2 in HT22 cells after being infected by lentiviruses carrying ASCT2, shASCT2, or shSRC [triplicate in RT-qPCR repeat, **p < 0.01, control vs. over-expressed (OverExp); *p < 0.05, control vs. down-expressed (DownExp); unpaired t-test]. (F) Cell uptake of L-serine, D-serine, and L-glutamine after being infected by lentiviruses carrying ASCT2, shASCT2, or shSRC. (n = 3, ***p < 0.0001, control vs. OverExp and control vs. DownExp; unpaired t-test). (G–I) SRR expression unchanged after CSDS. Relative Srr mRNA expression (n = 5; unpaired t-test) (G); Representative immunoblot (H); quantification of Srr protein expression (n = 8, unpaired t-test) (I).