Table E1.
Flow cytometric measurement of dendritic cell markers after 7 days of culture and after a further 24 hours of incubation in LPS
| Marker | Day 7 (%) | Day 8, LPS 0.1 (%) | Day 8, LPS 1.0 (%) | MFI day 7 | MFI day 8, LPS 0.1 | MFI day 8, LPS 1.0 |
|---|---|---|---|---|---|---|
| CD86 | 96 ± 4 | 99 ± 2 | 98 ± 2 | 6,275 ± 2,086 | 15,714 ± 4,383† | 15,728 ± 4,637‡ |
| CD40 | 91 ± 8 | 92 ± 1 | 96 ± 3 | 708 ± 477 | 564 ± 141 | 1,289 ± 6,964* |
| HLA-DR | 96 ± 6 | 99 ± 1 | 98 ± 3 | 1,198 ± 661 | 1,289 ± 690 | 1,754 ± 877 |
| PD-L1 | 34 ± 18 | 69 ± 26* | 77 ± 28† | 153 ± 85 | 401 ± 328 | 549 ± 377‡ |
| CD80 | 61 ± 32 | 83 ± 14 | 83 ± 12 | 412 ± 206 | 617 ± 199 | 745 ± 231† |
| CD1a | 62 ± 14 | 37 ± 20* | 34 ± 22† | 742 ± 363 | 273 ± 109† | 409 ± 378* |
| CD14 | 2 ± 3 | 2 ± 2 | 2 ± 2 | 16 ± 22 | 9 ± 36 | 3 ± 43 |
| CD11c | 96 ± 4 | 99 ± 1 | 97 ± 4 | 5,517 ± 1,280 | 6,530 ± 1,890 | 7,384 ± 2,987 |
Data are presented as means ± SDs. CD86, CD40, HLA-DR, and CD11c were expressed by greater than 90% of cells at day 7. CD86 and CD40 increased in MFI after 24 hours of LPS stimulation. CD80 percentage showed a nonsignificant increase after LPS and a significant change in MFI after incubation in the higher LPS concentration. The immature dendritic cell marker CD1a was expressed by 61% of cells at day 7, but both percentage of positive cells and MFI decreased after incubation in LPS. The monocyte marker CD14 (expressed by all cells at the start of the culture period) was expressed at background levels at day 7 and was not altered by LPS stimulation. See the Methods section for culture details.
One-way ANOVA (Kruskal-Wallis test with the Dunn posttest) showed significant P values, as indicated: *P < .05, †P < .01, and ‡P < .001 compared with the value on day 7.